GENERATION OF REACTIVE OXYGEN INTERMEDIATES IN RELATION TO SHORT-COURSE CHEMOTHERAPY IN CHILDREN SUFFERING FROM PULMONARY TUBERCULOSIS

Functional status of phagocytes in relation to 6 months' short course chemotherapy of tuberculosis was studied with special reference to ant igen-5 (Ag-5) of Mycobacterium tuberculosis. Phagocytic capacity and o verall generation of reactive oxygen intermediates (ROI) measured as c hemiluminescence (CL) response was studied in peripheral blood monocyt es obtained from 20 children with pulmonary tuberculosis (PTb) and was compared with 20, age and sex matched, controls. In untreated childre n the CL response (mean+/-SD) with latex (0.90+/-0.08) and Ag-5 (0.99/-0.11) was found to be severely depressed compared with controls (Lat ex, 19.2+/-5.10; Ag-5, 48.12+/-7.10). A significant improvement was se en in response to both the stimulants (Latex, 15.20+/-1.50; Ag-5, 28.1 5+/-7.32) after 3 months of therapy and became equal to, or slightly h igher than, the values obtained with controls by the end of therapy. T his study suggests that newly diagnosed untreated PTb is associated wi th an in vitro antigen specific compromise of phagocytic capacity and oxidative metabolic burst activity that is gradually and progressively corrected during the course of effective short course chemotherapy. I n addition, Ag-5 is a potent stimulator of monocytes/macrophages; its potency being far greater than latex.