IMMUNOLOGICAL DETECTION OF ISOFORMS OF THE SOMATOSTATIN RECEPTOR SUBTYPE, SSTR2

Somatostatin (SRIF) induces its diverse physiological actions through interactions with different receptor subtypes. Multiple SRIF receptor subtypes have recently been cloned. To analyze the physical properties of receptor subtype SSTR2, two different peptide-directed antibodies were generated against SSTR2. Antibody ''2e3,'' directed against the p eptide SSCTINWPGESGAWTT (residues 191-206), corresponding to a region in the predicted third extracellular domain of mouse SSTR2, and antibo dy ''2i4,'' directed against the peptide SGTEDGERSDS (residues 333-343 ) from the predicted cytoplasmic tail of mouse SSTR2, were developed. In Chinese hamster ovary (CHO) cells stably expressing the mouse SSTR2 gene (CHOB), the antibody 2e3 recognized specifically a protein of 93 -kDa protein by immunoblotting. No specific immunoreactivity was detec ted by 2e3 in nontransfected CHO cells or CHO cells stably expressing vector alone or human SSTR1 or mouse SSTR3 genes. The antibody 2i4 spe cifically immunoprecipitated SSTR2 solubilized from CHOB cells that co uld be labeled with the SSTR2-specific ligand I-125-MK-678. Furthermor e, both 2e3 and 2i4 specifically immunoprecipitated 93-kDa [S-35]methi onine-labeled proteins from CHOB cells, indicating that they recognize the same proteins. In contrast to studies in CHOB cells, immunoblotti ng studies showed that 2e3 detected specifically a single 148-kDa prot ein from different regions of the rat brain that have previously been shown to express high levels of SSTR2 mRNA and SR[F receptors with hig h affinity for I-125-MK-678. In contrast, no immunoreactivity was dete cted in rat kidney, liver, or lung, which do not express SSTR2. No 93- kDa protein was detected specifically in the rat brain. The 148-kDa pr otein detected by 2e3 is an SRIF receptor because 2e3 and 2i4 specific ally immunoprecipitated solubilized rat brain SR[F receptors that coul d be reversibly labeled with I-125-MK-678. As in rat brain, 2e3 intera cted specifically with a single 148-kDa protein in rat pituitary, in t he rat pancreatic cell line AR42J, and in the HEK 293 cell line derive d from human kidney, all of which express SSTR2 mRNA and SRIF receptor s with high affinity for I-125-MK-678. These findings indicate that ra t brain and pituitary, as well as a pancreatic and a kidney cell line, express primarily a form of SSTR2 different from CHOB cells. The mult iple forms of SSTR2 may result from differential post translational pr ocessing of SSTR2 because 2e3 immunoprecipitated 41-kDa in vitro trans lation products generated from mRNA extracted from CHOB and AR42J cell s. This 41-kDa protein has the predicted size of unprocessed SSTR2. Th ese results demonstrate that 2e3 and 2i4 antibodies interact specifica lly with SSTR2. Detection of two different size proteins by the SSTR2 peptide-directed antibodies suggests the existence of multiple forms o f SSTR2.