UBIQUINONE-10 PROTECTS NEURONS FROM VIRUS-INDUCED DEGENERATION

Cultured neurons from rat dorsal root ganglia and cerebral cortex were infected with Sendai virus, which gives a productive replication with lysis of most neurons, and with the RW strain of mumps virus, which u ndergoes defective replication causing degeneration of only 30-40% of the neurons within 5 days after initial infection. In Sendai virus-inf ected cells the amount of polyisoprenoid lipids was enhanced. In mumps virus-infected cultures there were transient reductions in the conten ts of cholesterol, dolichol, and ubiquinone-9 in the cultures, whereas the reduction in the ubiquinone-10 level was progressive, reaching 20 % of its original value 21 days after infection. Treatment of mumps vi rus-infected cultures with ubiquinone-10 protected the neurons from de generation, whereas no effects were observed on exposure to ubiquinone -9. Linolenic acid (18:3) and arachidonic acid (20:4), but not myristi c acid (14:0) and palmitic acid (16:0), also had significant neuroprot ective effects.