BACULOVIRUS EXPRESSION OF NONSTRUCTURAL PROTEIN-NS2 AND CORE PROTEIN-VP7 OF AFRICAN HORSESICKNESS VIRUS SEROTYPE-3 AND THEIR USE AS ANTIGENS IN AN INDIRECT ELISA

Authors
BREMER CW DUPLESSIS DH VANDIJK AA
Citation
Cw. Bremer et al., BACULOVIRUS EXPRESSION OF NONSTRUCTURAL PROTEIN-NS2 AND CORE PROTEIN-VP7 OF AFRICAN HORSESICKNESS VIRUS SEROTYPE-3 AND THEIR USE AS ANTIGENS IN AN INDIRECT ELISA, Journal of virological methods, 48(2-3), 1994, pp. 245-256
Citations number
35
Categorie Soggetti
Virology,"Biochemical Research Methods
Journal title
Journal of virological methods → ACNP
ISSN journal
01660934
Volume
48
Issue
2-3
Year of publication
1994
Pages
245 - 256
Database
ISI
SICI code
0166-0934(1994)48:2-3<245:BEONPA>2.0.ZU;2-L
Abstract
Non-structural protein NS2 and core protein VP7 of African horsesickne ss virus serotype 3 (AHSV3) were expressed in Spodoptera frugiperda ce lls by recombinant baculoviruses containing the relevant genes. These proteins were purified and analysed by polyacrylamide gel electrophore sis and Western blot. NS2 and VP7 were used separately as antigens in an indirect ELISA for the detection of AHSV antibodies. Both antigens cross-reacted with hyperimmune guinea-pig antisera to infected cell ly sates of all nine known AHSV serotypes and to antisera obtained from h orses immunized with attenuated virus of seven AHSV serotypes.