PROTECTIVE EFFECT OF ALPHA-TOCOPHEROL ON BRAIN-CELL MEMBRANE-FUNCTIONDURING CEREBRAL CORTICAL HYPOXIA IN NEWBORN PIGLETS

Protective effect of alpha-tocopherol on the structure and function of brain cell membranes was investigated by measuring Na+,K+-ATPase acti vity and products of lipid peroxidation (fluorescent compounds) in bra in cell membranes obtained from newborn piglets. Four groups of anesth etized, ventilated piglets were studied: five hypoxic piglets and five normoxic piglets were pretreated with free alpha-tocopherol (20 mg/kg /dose i.m.), five additional hypoxic piglets received i.m. placebo and five normoxic piglets served as control. Placebo and alpha-tocopherol were given 48 and 3 h prior to onset of hypoxia. Hypoxic hypoxia was induced and cerebral hypoxia was documented as a decrease in the ratio of phosphocreatine to inorganic phosphate (PCr/P-i) using P-31 NMR sp ectroscopy. PCr/P-i decreased from baseline of 2.62 +/- 0.54 to 1.05 /- 0.27 in alpha-tocopherol-pretreated and from 2.44 +/- 0.48 to 1.14 +/- 0.30 in the placebo-pretreated group during hypoxia. Na+,K+-ATPase activity was unchanged in both normoxic and hypoxic alpha-tocopherol- pretreated groups. However, in placebo-pretreated hypoxic group, Na+,K +-ATPase activity decreased as compared with control (44.9 +/- 9.7 vs. 61.8 +/- 5.7 pmol P-i/mg protein/h, P < 0.005). The level of fluoresc ent compounds increased in placebo-pretreated but not in alpha-tocophe rol-pretreatcd group as compared with control. During hypoxia, serum a lpha-tocopherol levels were higher in alpha-tocopherol-pretreated grou ps as compared with placebo-pretreated hypoxic group. The present data indicates that alpha-tocopherol protects brain cell membranes in newb orn piglets from lipid peroxidative damage during tissue hypoxia proba bly by being incorporated in cell membrane and also as circulating ant ioxidant.