GROWTH-ARRESTED DEPENDENCE OF ALDOLASE A L-TYPE MESSENGER-RNA EXPRESSION IN RODENT CELL-LINES

Two ubiquitous (L- and F-type) and one muscle-specific (M-type) mRNA s pecies are generated by the human aldolase A gene. Despite the high de gree of sequence similarities in the promoter region between human and rodents, no L-type mRNA expression has yet been found in the latter. Here we demonstrate that L-type aldolase A mRNA is expressed during th e differentiation of mouse myogenic C2.7 and rat oligodendrocyte precu rsor CEINGE C13 cells. The L-type mRNA expression is increased during differentiation and is associated with cell-growth arrest caused by no codazole treatment or serum deprivation in C2.7 and CEINGE C13 cells, respectively. The L-type aldolase A mRNA is correctly processed at the L1-L2 junction. (C) 1994 Academic Press, Inc.