PRODUCTION OF RECOMBINANT POLYPEPTIDES OF HERPES-SIMPLEX VIRUS TYPE-1AND TYPE-2

Authors
ZVONAREV AY SHATALIN KY KLICHKO VI KARASEVA EV KULYAKINA MN
Citation
Ay. Zvonarev et al., PRODUCTION OF RECOMBINANT POLYPEPTIDES OF HERPES-SIMPLEX VIRUS TYPE-1AND TYPE-2, Voprosy virusologii, 39(3), 1994, pp. 110-113
Citations number
22
Categorie Soggetti
Virology
Journal title
Voprosy virusologii → ACNP
ISSN journal
05074088
Volume
39
Issue
3
Year of publication
1994
Pages
110 - 113
Database
ISI
SICI code
0507-4088(1994)39:3<110:PORPOH>2.0.ZU;2-7
Abstract
The genes IE175, IE63, IE68, IE12, UL29 and UL19 of herpes simplex vir us types 1 and 2 were cloned. Fragments of these genes were cloned int o open reading frame expression vector pEX1-3. Recombinant proteins co ntaining amino acid sequences of ICP4, ICP27, ICP22, ICP47, major DNA- binding protein and major capsid protein were expressed in E. coli cel ls by fusion with cro-beta-galactosidase proteins.