Five overlapping lambda EMBL-clones, containing the complete bovine la
ctoferrin gene (LTF), have been used to map this gene by fluorescence
in situ hybridization to bovine Chromosome (Chr) band 22q24. Primers d
erived from promoter and exon I sequences were applied in polymerase c
hain reactions (PCRs) to DNA samples of a previously characterized pan
el of somatic cell hybrid lines, allowing the assignment of the bovine
lactoferrin locus to syntenic group U12. These results permit the ass
ignment of syntenic group U12 to bovine Chr 22.