Jms. Mallols et al., CONCENTRATION GRADIENT PERTURBATIONS IN MICELLAR LIQUID-CHROMATOGRAPHY WITH THERMAL LENS SPECTROPHOTOMETRIC DETECTION, Analytical letters, 27(10), 1994, pp. 2011-2026
In HPLC with a sodium dodecyl sulphate micellar mobile phase, a pertur
bation of the sodium ion equilibrium gives rise to a refractive index
gradient associated to a concentration gradient. This produces a loss
of sensitivity and an increase of noise at a reproducible value of the
retention time when thermal lens spectrometric detection is used. Bas
eline perturbations as this one produced by retained components of the
mobile phase can overlap with the peaks of the analytes.