DETECTION OF MULTIPLE VIRULENCE-ASSOCIATED GENES OF LISTERIA-MONOCYTOGENES BY PCR IN ARTIFICIALLY CONTAMINATED MILK SAMPLES

Authors
COORAY KJ NISHIBORI T XIONG HB MATSUYAMA T FUJITA M MITSUYAMA M
Citation
Kj. Cooray et al., DETECTION OF MULTIPLE VIRULENCE-ASSOCIATED GENES OF LISTERIA-MONOCYTOGENES BY PCR IN ARTIFICIALLY CONTAMINATED MILK SAMPLES, Applied and environmental microbiology, 60(8), 1994, pp. 3023-3026
Citations number
21
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
60
Issue
8
Year of publication
1994
Pages
3023 - 3026
Database
ISI
SICI code
0099-2240(1994)60:8<3023:DOMVGO>2.0.ZU;2-6
Abstract
The inhibitory effect of milk in the PCR detection of Listeria monocyt ogenes could be overcome by washing the contaminated milk sample with phosphate-buffered saline and concentrating the bacteria to 1/10 of th e original volume. In order to avoid a possible failure in the detecti on of virulent L. monocytogenes, a one-step procedure which enabled de monstration of three virulence-associated genes, prfA, hlyA, and plcB, simultaneously in a single PCR mixture was developed.