OPTIMIZATION OF ALPHA-AMYLASE AND GLUCOAMYLASE PRODUCTION BY RECOMBINANT STRAINS OF SACCHAROMYCES-CEREVISIAE

Authors
DAGUANNO VS PRETORIUS IS
Citation
Vs. Daguanno et Is. Pretorius, OPTIMIZATION OF ALPHA-AMYLASE AND GLUCOAMYLASE PRODUCTION BY RECOMBINANT STRAINS OF SACCHAROMYCES-CEREVISIAE, Biotechnology letters, 16(7), 1994, pp. 727-732
Citations number
13
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
16
Issue
7
Year of publication
1994
Pages
727 - 732
Database
ISI
SICI code
0141-5492(1994)16:7<727:OOAAGP>2.0.ZU;2-S
Abstract
Replacement of the regulatory sequence of the Bacillus amyloliquefacie ns alpha-amylase gene (AMY1) by the yeast alcohol dehydrogenase gene p romoter (ADC1(P)) resulted in increased levels of extracellular alpha- amylase production in Saccharomyces cerevisiae. Negative regulation of glucoamylase synthesis by the STA10-encoded repressor was alleviated by replacing the native STA2 gene promoter from S. cerevisiae var. dia staticus with ADC1(P). Enhanced degradation of starch was achieved whe n the modified versions of the AMY1 and STA2 genes were introduced joi ntly into S. cerevisiae.