Vs. Daguanno et Is. Pretorius, OPTIMIZATION OF ALPHA-AMYLASE AND GLUCOAMYLASE PRODUCTION BY RECOMBINANT STRAINS OF SACCHAROMYCES-CEREVISIAE, Biotechnology letters, 16(7), 1994, pp. 727-732
Replacement of the regulatory sequence of the Bacillus amyloliquefacie
ns alpha-amylase gene (AMY1) by the yeast alcohol dehydrogenase gene p
romoter (ADC1(P)) resulted in increased levels of extracellular alpha-
amylase production in Saccharomyces cerevisiae. Negative regulation of
glucoamylase synthesis by the STA10-encoded repressor was alleviated
by replacing the native STA2 gene promoter from S. cerevisiae var. dia
staticus with ADC1(P). Enhanced degradation of starch was achieved whe
n the modified versions of the AMY1 and STA2 genes were introduced joi
ntly into S. cerevisiae.