DIFFERENTIAL INDUCTION OF FOS PROTEIN AND A FOS-RELATED ANTIGEN FOLLOWING ACUTE AND REPEATED COCAINE ADMINISTRATION

The present study examined the effects of a single and five once daily injections of cocaine on the expression of c-fos mRNA, Fos protein an d Fos-related antigens (Fra) in the striatum. A single injection (40 m g/kg, i.p.), which induces locomotion, increased the expression of c-f os mRNA, Fos protein and a 35 kDa Fra. In contrast, five injections (4 0 mg/kg, i.p.) given once a day, which induces even more behavioral st imulation, diminished the increase in c-fos mRNA and Fos protein expre ssion. However, the cocaine-induced Fra expression was sustained and n ot reduced after the five injections. The results demonstrate that coc aine-induced expression of the Fos/Fra gene family is dynamic and the profile of gene transcription and translation in the striatum changes when animals are behaviorally more sensitive to cocaine.