CLONING AND EXPRESSION OF A CDNA FOR RAT PROSTAGLANDIN-F2-ALPHA RECEPTOR

Authors
KITANAKA J HASIMOTO H SUGIMOTO Y NEGISHI M AINO H GOTOH M UCHIKAWA A BABA A
Citation
J. Kitanaka et al., CLONING AND EXPRESSION OF A CDNA FOR RAT PROSTAGLANDIN-F2-ALPHA RECEPTOR, Prostaglandins, 48(1), 1994, pp. 31-41
Citations number
25
Categorie Soggetti
Endocrynology & Metabolism
Journal title
Prostaglandins → ACNP
ISSN journal
00906980
Volume
48
Issue
1
Year of publication
1994
Pages
31 - 41
Database
ISI
SICI code
0090-6980(1994)48:1<31:CAEOAC>2.0.ZU;2-X
Abstract
We have cloned a cDNA for rat prostaglandin (PG) F-2 alpha receptor fr om cultured rat astrocytes. The cDNA encodes a polypeptide of 366 amin o acids with seven putative transmembrane domains. Specific binding of [H-3]PGF(2 alpha) in membranes of COS-7 cells transfected with the cD NA was displaced with unlabeled PGs in the order of PGF(2 alpha) > PGD (2) > PGE(2) > PGI(2). In the cDNA-transfected LLC-PK1 cells, PGF(2 al pha) stimulated phosphoinositide hydrolysis. A significant 4.7-kb mRNA transcript was detected in cultured rat astrocytes and whole brain an d pregnant ovary of adult rats by Northern blot analysis.