SEPARATION OF MONOPHOSPHATE, DIPHOSPHATE, AND TRIPHOSPHATE NUCLEOTIDES BY CYTOSINE SUBSTITUTED, SILICA-BOUND SAPPHYRIN SOLID SUPPORTS

A cytosine-substituted sapphyrin when used as a silica gel bound HPLC solid support effectively separates guanosine 5'-mono-, di-, and triph osphate from a mixture of the mono-, di- and triphosphates of cytidine , uridine, adenosine, and guanosine, respectively under isocratic cond itions at pH 7. In addition, using this same solid support, cytidine 5 '-monophosphate, guanosine 5'-monophosphate, adenosine 5'-monophosphat e and uridine 5'-monophosphate are all readily separated from each oth er under similar HPLC conditions.