SYNTHESIS OF CELL-WALL GALACTANS FROM FLAX (LINUM-USITATISSIMUM L) SUSPENSION-CULTURED CELLS

Authors
Citation
F. Goubet et C. Morvan, SYNTHESIS OF CELL-WALL GALACTANS FROM FLAX (LINUM-USITATISSIMUM L) SUSPENSION-CULTURED CELLS, Plant and Cell Physiology, 35(5), 1994, pp. 719-727
Citations number
22
Categorie Soggetti
Plant Sciences
Journal title
ISSN journal
00320781
Volume
35
Issue
5
Year of publication
1994
Pages
719 - 727
Database
ISI
SICI code
0032-0781(1994)35:5<719:SOCGFF>2.0.ZU;2-D
Abstract
Cell walls of flax suspension-cultured cells contain both beta 1 --> 4 galactan (most of which can be extracted with boiling water) and beta 1 --> 3, beta 1 --> 6 galactan (most of which can be solubilized with alkali). The relative level of beta 1 --> 4 galactan in the cell wall s is maximal during the lag phase, accounting for an average of 8% of the dry cell-wall mass, whereas the relative level of beta 1 --> 3, be ta 1 --> 6 galactan increases throughout growth, reaching a maximum (u p to 20%) during the exponential phase. Two peaks of galactan synthase activity can be detected that catalyze the synthesis of beta 1 --> 4 galactan at pH 8 during the lag phase and the synthesis of beta 1 --> 3, beta 1 --> 6 galactan at pH 5 during the growth phase. At the end o f the growth phase both activities are negligible.