CAPILLARY GC MS CHARACTERIZATION OF FATTY-ACIDS FROM INDIGENOUS SILKWORM OIL/

The fatty acids contained in indigenous silkworm oil were determined b y capillary gas chromatography/mass spectrometry after their derivatiz ation. Five different derivatization techniques were investigated usin g cold on-column and split injection systems. The separations were per formed on three polar fused-silica capillary columns, coated with FFAP , Carbowax 20M, and SP-2340 stationary phases. The final results were resumed in a table. The indigenous silkworm oil is shown to contain 21 -23% palmitic acid, 0.2-0.5% palmitoleic acid, 5-7% stearic acid, 31-3 4% oleic acid, 6-8% linoleic acid, and 30-32% linolenic acid. Traces o f lauric, myristic, arachic, and behenic acids were also detected. (C) 1997 John Wiley & Sons, Inc.