IMPROVED DISCRIMINATION OF BOVINE CLASS-II DR-BETA-CHAINS POLYMORPHISMS USING IMMUNOBLOTTING

Authors
KNOWLES G DUTIA BM GLASS EJ MACCARTHYMORROGH L SPOONER RL HOPKINS J
Citation
G. Knowles et al., IMPROVED DISCRIMINATION OF BOVINE CLASS-II DR-BETA-CHAINS POLYMORPHISMS USING IMMUNOBLOTTING, Animal genetics, 25(3), 1994, pp. 129-131
Citations number
10
Categorie Soggetti
Genetics & Heredity","Veterinary Sciences
Journal title
Animal genetics → ACNP
ISSN journal
02689146
Volume
25
Issue
3
Year of publication
1994
Pages
129 - 131
Database
ISI
SICI code
0268-9146(1994)25:3<129:IDOBCD>2.0.ZU;2-Z
Abstract
An immunoblotting technique is reported that reveals electrophoretic v ariants in the beta-chains of class II antigens of the bovine major hi stocompatibility complex. One monoclonal antibody, mAb VPM57, reacted on immunoblots with an epitope present in approximately half of the ha plotypes investigated. This reagent is especially useful in discrimina ting electrophoretic variants that have similar isoelectric points.