A. Zhiri et P. Belichard, REVERSED-PHASE LIQUID-CHROMATOGRAPHIC ANALYSIS OF COENZYME-Q10 AND STABILITY STUDY IN HUMAN PLASMA, Journal of liquid chromatography, 17(12), 1994, pp. 2633-2640
The objectives of this study were to design and validate a method for
the assay of coenzyme Q10 (CoQ10) in human plasma and to evaluate the
stability of CoQ10 in several conditions currently observed for routin
e determinations. CoQ10 was extracted from plasma with n-hexane after
dissociation from lipoproteins with methanol. Ethoxy-CoQ10 was used as
internal standard. CoQ10 was isolated and then quantitated by high pe
rformance liquid chromatography (HPLC) using a binary gradient. Linear
ity (r = 0.9999), recovery (97 %) and intra- and inter-run precision (
1.7 and 2.0 % respectively) appeared to be satisfactory. The stability
of CoQ10 in crude plasma was tested under various conditions (i.e. si
x hours at room temperature, freezing and thawing, storage for up to 2
4 weeks at - 20 degrees C), the stability of CoQ10 in n-hexane extract
s was also tested (24 hours in autosampler rack at room temperature).
CoQ10 content was found to be unaffected by any of the tested conditio
ns.