INFLUENCE OF SAMPLE RECOVERY TECHNIQUES ON DETECTION OF BIOGENIC-AMINES IN THE RAT HOOKWORM NIPPOSTRONGYLUS-BRASILIENSIS

Biogenic amines in Nematoda have previously been found by means of hig h-performance liquid chromatography (HPLC) equipped with various detec tors. The methods of sample preparation have differed widely. In this study, we tested the influence of experimental conditions (temperature and duration of exposure to light, centrifugation or individual recov ery by pipetting each worm, and saccharose gradient versus physiologic al saline) on catecholamines and indolamines content in Nippostrongylu s brasiliensis at various developmental stages (larvae and adults). Am ines were determined by liquid chromatography with electrochemical det ection.