MATRIX SOLID-PHASE DISPERSION AS A MULTIRESIDUE EXTRACTION TECHNIQUE FOR BETA-AGONISTS IN BOVINE LIVER-TISSUE

A procedure for the extraction of salbutamol including conjugated form s of the drug, from liver samples, is described. It combines matrix so lid-phase dispersion with radioimmunoassay for the measurement of salb utamol residues at the sub-ppb level. Inter- and intra-assay validatio n, carried out on fortified liver samples, show good recoveries over t he range 1-5 ppb of salbutamol. An enzyme hydrolysis procedure was opt imized for the deconjugation of incurred residue. The developed proced ure is shown to be suitable for the extraction and determination of ot her P-agonists such as clenbuterol, mabuterol, terbutaline, and cimate rol at residue levels of less than 1 ng g(-1).