DETERMINATION OF CEFTRIAXONE IN AQUEOUS-HUMOR AND SERUM SAMPLES BY DIFFERENTIAL-PULSE ADSORPTIVE STRIPPING VOLTAMMETRY

Differential-pulse adsorptive stripping voltammetry was used to determ ine ceftriaxone in serum and aqueous humour samples. The method involv ed extraction of the ceftriaxone from serum samples with an Amberlite XAD-2 column followed by elution with methanol. The recovery was 97.6% with a relative standard deviation of 3.3% at a ceftriaxone concentra tion of 90.9 mu g l(-1). Peak currents of ceftriaxone were measured wi th a hanging mercury drop electrode at -0.78 V versus an Ag-AgCl refer ence electrode in pH 3.0 Britton-Robinson buffer. The calibration grap h was linear from 0.02 to 1300 mu g l(-1). The method was applied to c ataract cases and ceftriaxone levels were measured in aqueous humour a nd serum samples from patients who had received 1 or 2 g of ceftriaxon e intravenously. Aqueous humour was added to the polarographic cell di rectly. The amounts of ceftriaxone in the aqueous humour and serum sam ples with respect to time were measured. The pharmacokinetic profiles for 1 and 2 g were compared.