GENE SEQUENCE OF RECA(-CHOLERAE() AND CONSTRUCTION OF RECA MUTANTS OFVIBRIO)

Authors
STROEHER UH LECH AJ MANNING PA
Citation
Uh. Stroeher et al., GENE SEQUENCE OF RECA(-CHOLERAE() AND CONSTRUCTION OF RECA MUTANTS OFVIBRIO), MGG. Molecular & general genetics, 244(3), 1994, pp. 295-302
Citations number
53
Categorie Soggetti
Genetics & Heredity",Biology
Journal title
MGG. Molecular & general genetics → ACNP
ISSN journal
00268925
Volume
244
Issue
3
Year of publication
1994
Pages
295 - 302
Database
ISI
SICI code
0026-8925(1994)244:3<295:GSORAC>2.0.ZU;2-O
Abstract
The recA(+) gene of Vibrio cholerae O1 has been cloned, its nucleotide sequence determined and the product characterized. A deletion mutatio n was constructed in the recA gene and mutants showed the typical sens itivity to UV and to DNA-damaging agents, as well as an inability to m ediate homologous DNA recombination. The chromosomal recA deletion mut ants in V. cholerae do not show altered virulence in the infant mouse cholera model and are thus ideal strains for use in complementation st udies.