GROWTH AND OSMOREGULATION OF BOEKELOVIA-HOOGLANDII IN RELATION TO SALINITY

Citation
S. Fujii et Ja. Hellebust, GROWTH AND OSMOREGULATION OF BOEKELOVIA-HOOGLANDII IN RELATION TO SALINITY, Canadian journal of botany, 72(6), 1994, pp. 823-828
Citations number
17
Categorie Soggetti
Plant Sciences
Journal title
ISSN journal
00084026
Volume
72
Issue
6
Year of publication
1994
Pages
823 - 828
Database
ISI
SICI code
0008-4026(1994)72:6<823:GAOOBI>2.0.ZU;2-B
Abstract
The growth and osmoregulation of Boekelovia hooglandii Nicolai et Baas Becking (Chrysophyceae) was investigated as a function of salinity. T his chromophyte microflagellate was found to be euryhaline and also to require sodium for growth. The optimum sodium chloride concentration for growth was 0.2-0.4 M, and growth was severely inhibited in media w ith sodium chloride concentrations above 1 M. Using gas chromatography - mass spectroscopy analysis of cell extracts, it was established tha t the alga contains D-mannitol, myo-inositol, as well as the inositol derivative, cyclohexanetetrol. The content of cyclohexanetetrol and ma nnitol increased with increased salinity, while the content of inosito l remained almost constant. Potassium and magnesium were the major int racellular cations. However, the content of cations and amino acids sh owed only minor increases with salinity over a wide salinity range. Th e contents of cyclohexanetetrol and mannitol increased rapidly when ce lls were subjected to hypertonic shocks. It was concluded that B. hoog landii utilizes cyclohexanetetrol and mannitol as osmoregulatory subst ances.