J. Clark et al., IDENTIFICATION OF NOVEL GENES, SYT AND SSX, INVOLVED IN THE T(X18)(P11.2Q11.2) TRANSLOCATION FOUND IN HUMAN SYNOVIAL SARCOMA, Nature genetics, 7(4), 1994, pp. 502-508
Human synovial sarcomas contain a recurrent and specific chromosomal t
ranslocation t(X;18)(p11.2;q11.2). By screening a synovial sarcoma cDN
A library with a yeast artificial chromosome spanning the X chromosome
breakpoint, we have indentified a hybrid transcript that contains 5'
sequences (designated SYT) mapping to chromosome 18 and 3' sequences (
designated SSX) mapping to chromosome X. An SM probe detected genomic
rearrangements in 10/13 synovial sarcomas. Sequencing of cDNA clones s
hows that the normal SM gene encodes a protein rich in glutamine, prol
ine and glycine, and indicates that in synovial sarcoma rearrangement
of the SYT gene results in the formation of an STT-SSX fusion protein.
Both SYT and SSX failed to exhibit significant homology to known gene
sequences.