A T7 PROMOTER VECTOR WITH A TRANSCRIPTIONAL TERMINATOR FOR STRINGENT EXPRESSION OF FOREIGN GENES

Authors
NISHIHARA T IWABUCHI T NOHNO T
Citation
T. Nishihara et al., A T7 PROMOTER VECTOR WITH A TRANSCRIPTIONAL TERMINATOR FOR STRINGENT EXPRESSION OF FOREIGN GENES, Gene, 145(1), 1994, pp. 145-146
Citations number
11
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
145
Issue
1
Year of publication
1994
Pages
145 - 146
Database
ISI
SICI code
0378-1119(1994)145:1<145:ATPVWA>2.0.ZU;2-G
Abstract
An expression vector, pKK233-2 [Amann and Brosius, Gene 40 (1985) 183- 190], was modified by replacing the trc promoter with the T7 phi 10 pr omoter and introducing a strong transcriptional terminator upstream fr om this promoter. This modification successfully suppressed background transcription and resulted in strict dependence upon induction.