6-FLUOROPYRIDOXOL - A NOVEL PROBE OF CELLULAR PH USING F-19 NMR-SPECTROSCOPY

Authors
MEHTA VD KULKARNI PV MASON RP CONSTANTINESCU A ARAVIND S GOOMER N ANTICH PP
Citation
Vd. Mehta et al., 6-FLUOROPYRIDOXOL - A NOVEL PROBE OF CELLULAR PH USING F-19 NMR-SPECTROSCOPY, FEBS letters, 349(2), 1994, pp. 234-238
Citations number
27
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
349
Issue
2
Year of publication
1994
Pages
234 - 238
Database
ISI
SICI code
0014-5793(1994)349:2<234:6-ANPO>2.0.ZU;2-C
Abstract
6-Fluoropyridoxol was evaluated as an intracellular pH indicator. This molecule exhibits exceptional sensitivity to changes in pH (similar t o 10 ppm acid/base shift) and a pK(a) similar to 8.2 appropriate for p hysiological investigations. Using F-19 NMR spectroscopy we determined both intra- and extracellular pH in whole blood and confirmed the mea surements using traditional techniques: ion-electrodes and P-31 NMR sp ectroscopy.