PRELIMINARY CRYSTALLOGRAPHIC STUDY OF ESCHERICHIA-COLI RUVC PROTEIN -AN ENDONUCLEASE SPECIFIC FOR HOLLIDAY JUNCTIONS

Authors
ARIYOSHI M VASSYLYEV DG IWASAKI H FUJISHIMA A SHINAGAWA H MORIKAWA K
Citation
M. Ariyoshi et al., PRELIMINARY CRYSTALLOGRAPHIC STUDY OF ESCHERICHIA-COLI RUVC PROTEIN -AN ENDONUCLEASE SPECIFIC FOR HOLLIDAY JUNCTIONS, Journal of Molecular Biology, 241(2), 1994, pp. 281-282
Citations number
10
Categorie Soggetti
Biology
Journal title
Journal of Molecular Biology → ACNP
ISSN journal
00222836
Volume
241
Issue
2
Year of publication
1994
Pages
281 - 282
Database
ISI
SICI code
0022-2836(1994)241:2<281:PCSOER>2.0.ZU;2-2
Abstract
Single crystals of the RuvC protein, an Escherichia coli endonuclease specific for Holliday junctions, were grown by the microdialysis metho d. The crystals belong to the space group P2(1), with unit cell dimens ions a = 72.8 Angstrom, b = 139.6 Angstrom, c = 32.4 Angstrom and beta = 93.0 degrees, and contain four molecules in an asymmetric unit. Dif fraction data to a Bragg spacing of 2.5 Angstrom resolution has been o btained using a synchrotron X-ray source.