Se. Safford et al., SUPPRESSION OF FIBROSARCOMA METASTASIS BY ELEVATED EXPRESSION OF MANGANESE SUPEROXIDE-DISMUTASE, Cancer research, 54(16), 1994, pp. 4261-4265
A mouse fibrosarcoma cell line (FSa-II), which exhibits low endogenous
levels of manganese superoxide dismutase, was transfected with a huma
n manganese superoxide dismutase complementary DNA. Fifty clones were
screened for manganese superoxide dismutase activity by the superoxide
dismutase activity gel assay. Activity of the positive clones was mea
sured by the nitro blue tetrazolium reduction assay in the presence of
cyanide, Three cell lines exhibiting a range of activity were chosen
to be transplanted into syngeneic mice. The results indicated that the
metastasis rate for all transfected cells was significantly less than
that of control cells.