Ta. Titus, TISSUE-SPECIFIC GENE-EXPRESSION IN THE SMALLMOUTH SALAMANDER, AMBYSTOMA-TEXANUM (CAUDATA, AMBYSTOMATIDAE), Biochemical systematics and ecology, 22(5), 1994, pp. 477-489
Relative staining activity, heteropolymer assembly, and secondary isoz
ymes are described for gene products of 22 loci examined for expressio
n in eight tissues of Ambystoma texanum. Locus homologies based on tis
sue specificity and staining patterns in mitochondria-enriched liver f
ractions are proposed. Quaternary structure was inferred from polymorp
hisms resolved at seven loci. Gene expression was undetectable in 17 o
f the 176 tissue/locus comparisons. The phylogenetic pattern of change
s in gene number and the extensive variation in tissue-specific expres
sion among tetrapods suggest that these attributes will be useful as p
hylogenetic characters in groups less inclusive than tetrapods.