COMPARATIVE VOLTAMMETRIC STUDY OF 2,4-DINITROPHENOL (DNP), ALBUMIN AND DNP-ALBUMIN AS AN ANALYTICAL APPROACH TO THE USE OF DNP AS A UNIVERSAL LABEL IN IMMUNOELECTROCHEMICAL ASSAYS

Authors
COSTAGARCIA A FERNANDEZABEDUL MT TUNONBLANCO P
Citation
A. Costagarcia et al., COMPARATIVE VOLTAMMETRIC STUDY OF 2,4-DINITROPHENOL (DNP), ALBUMIN AND DNP-ALBUMIN AS AN ANALYTICAL APPROACH TO THE USE OF DNP AS A UNIVERSAL LABEL IN IMMUNOELECTROCHEMICAL ASSAYS, Talanta, 41(7), 1994, pp. 1191-1200
Citations number
31
Journal title
Talanta
ISSN journal
00399140 → ACNP
Volume
41
Issue
7
Year of publication
1994
Pages
1191 - 1200
Database
ISI
SICI code
0039-9140(1994)41:7<1191:CVSO2(>2.0.ZU;2-I
Abstract
A comparative electrochemical study of 2,4-dinitrophenol (DNP), albumi n and DNP-albumin has been carried out at a hanging mercury drop elect rode, in order to use DNP as a universal label in immunoelectrical ass ays. Several electrochemical techniques have been used. Differential p ulse voltammetry has proved to be the most suitable. Wide dynamic line ar ranges (more than three orders of magnitude for DNP-albumin) and lo w detection limits have been achieved (5 x 10(-10) M, 2 x 10(-10) M, 3 x 10(-12) M for DNP, albumin and DNP-albumin, respectively). Good rep roducibility has been obtained in all cases (R.S.D. < 2.2%).