HETEROLOGOUS SYSTEMS FOR EXPRESSION OF MAMMALIAN SULFOTRANSFERASES

Authors
VERONESE ME BURGESS W CRUICKSHANK D SANSOM L ZHU XY MCMANUS ME
Citation
Me. Veronese et al., HETEROLOGOUS SYSTEMS FOR EXPRESSION OF MAMMALIAN SULFOTRANSFERASES, Chemico-biological interactions, 92(1-3), 1994, pp. 77-85
Citations number
14
Categorie Soggetti
Toxicology,Biology,Chemistry,Biology
Journal title
Chemico-biological interactions → ACNP
ISSN journal
00092797
Volume
92
Issue
1-3
Year of publication
1994
Pages
77 - 85
Database
ISI
SICI code
0009-2797(1994)92:1-3<77:HSFEOM>2.0.ZU;2-Q
Abstract
This paper describes the use of both mammalian and bacterial expressio n systems as tools to study the structural and functional relationship s of proteins encoded by cDNAs to both rat and human aryl sulfotransfe rases. In particular, we describe the use of the mammalian COS cell sy stem for functional expression studies, and the use of Escherichia col i for the expression and purification of a sulfotransferase fusion pro tein suitable as an antigen for the generation of sulfotransferase ant ibodies.