HPLC DETERMINATION OF ENANTIOMERIC PURITY OF PROTECTED AMINO-ACID DERIVATIVES USED IN PEPTIDE-SYNTHESIS

An improved RP-HPLC method on ODS-Hypersil column with precolumn deriv atization with Marfey's reagent were used to monitor racemization in N -, C- and/or side-chain protected amino acid derivatives by separation of a series of new diastereoisomeric Marfey's compounds. Chromatograp hic samples were obtained by partial deprotection of different startin g materials. In a simple two-step procedure (deprotection and derivati zation) the compounds of amino acids formed stable diastereomeric deri vatives having facile resolutions.