HPLC ANALYSIS OF OXIDATION HAIR-DYES IN PERMANENT HAIR COLORANTS

The reversed-phase liquid chromatographic separation of several hair d yes (diamimes, aminophenols, phenols, etc.), using mobile phases conta ining 1,8-diaminoctane as new amine modifier and sodium heptansulfonat e, is described. The combined effect of the amine and of the alkylsulf onate enabled very good separation of all the dyes studied. The propos ed chromatographic system was found to be selective, rugged and theref ore suitable for the reliable quality control of commercial permanent colorants. A preliminary solid phase extraction (SPE) procedure using SCX sorbents, was found useful to enhance detectability of low concent rated dyes and to isolate phenol compounds from the basic dyes.