IDENTIFICATION OF VCR, A REPEATED SEQUENCE ASSOCIATED WITH A LOCUS ENCODING A HEMAGGLUTININ IN VIBRIO-CHOLERAE O1

Citation
A. Barker et al., IDENTIFICATION OF VCR, A REPEATED SEQUENCE ASSOCIATED WITH A LOCUS ENCODING A HEMAGGLUTININ IN VIBRIO-CHOLERAE O1, Journal of bacteriology, 176(17), 1994, pp. 5450-5458
Citations number
60
Categorie Soggetti
Microbiology
Journal title
ISSN journal
00219193
Volume
176
Issue
17
Year of publication
1994
Pages
5450 - 5458
Database
ISI
SICI code
0021-9193(1994)176:17<5450:IOVARS>2.0.ZU;2-9
Abstract
We have determined the nucleotide sequence of a 6.3-kb BamHI fragment of the chromosome of Vibrio cholerae 569B that includes the sequence o f the mannose-fucose-resistant hemagglutinin reported previously (V. L . Franzon, A. Barker, and P. A. Manning, Infect. Immun. 61:3032-3037, 1993). This region contains nine copies of a 124-bp direct repeat, her e named VCR, of imperfect dyad symmetry, that are shown by Southern hy bridization to occur at least 60 to 100 times in the V. cholerae 01 ch romosome. Large-scale chromosomal mapping suggests that the repeats ar e confined to about 10% of the chromosome. Related sequences are also found in non-01 V. cholerae but not in other members of the family Vib rionaceae. However, VCR is unrelated to other previously described rep etitive sequences.