Fl. Erdkamp et al., COMPARISON OF IMAGE (CAS-200) AND FLOW-CYTOMETRY DETERMINED DNA CONTENT OF PARAFFIN-EMBEDDED HODGKINS-DISEASE TISSUE, Hematologic pathology, 8(3), 1994, pp. 75-84
To assess the reliability of DNA estimation using image cytometry, dep
araffinized lymph nodes from 70 patients with Hodgkin's disease were e
xamined and the results obtained were compared with those from flow cy
tometry. Image analysis without discriminating between the various cel
l types, as found in Hodgkin's disease, revealed no separate aneuploid
peak. Selecting on morphologically defined nuclear types DNA aneuploi
dy was detected in 20% of the cases (14/70). The aneuploid populations
were limited to the population of nuclei defined as Reed-Sternberg (R
S)-like or medium-sized lymphocytes. Benign lymph nodes DNA aneuploidy
was not found in any of the controls. Comparison of DNA histograms ob
tained by image and flow cytometry showed aneuploid peaks using image
cytometry in 4 of 30 diploid and 10 of 40 aneuploid flow histograms. I
n conclusion, image analysis using the CAS 200 system as compared to f
low cytometry is more time-consuming and less sensitive to assess ploi
dy status, although it may provide extra information in some selected
cases. Evidence is obtained that DNA aneuploidy in Hodgkin's disease i
s preferentially expressed by cells with the RS/H-Eike and medium-size
d lymphocyte morphology.