ONE-HOUR DOWNWARD CAPILLARY BLOTTING OF RNA AT NEUTRAL PH

This report describes a setup for the downward capillary blotting of R NA with the use of 10X SSC as a transfer solution. The setup is compos ed of a stack of blotting papers, hybridization membrane, and agarose gel. Two layers of blotting paper connect the stack with two reservoir s containing transfer solution. Using this setup, blotting of RNA frag ments (<7.5 kb) can be completed in 1 h. If necessary, the blotting ti me can be expanded from 1 to 18 h without decrease in hybridization ef ficiency of RNA. (C) 1994 Academic Press, Inc.