A kinematic analysis was carried out to compare treadmill and overgrou
nd locomotion in horses at the trot. Stride variables and limb and tru
nk movements of 10 Dutch Warmblood horses were measured using the CODA
-3 gait analysis system. Overground recordings were made on a rubber g
round surface and on an asphalt track. Treadmill recordings were taken
after a controlled habituation programme and at the same velocities a
s measured overground. On asphalt, a shorthened stride duration and a
decreased vertical displacement of the withers were found compared wit
h those on rubber ground. On the treadmill, stance duration of the for
elimbs was longer than for either overground condition. Correspondingl
y, the hind limbs were placed earlier than the forelimbs in making dia
gonal ground contact in overground locomotion, but this changed to pre
ceding forelimbs on the treadmill. Both forelimbs and hind limbs were
moved more caudally during the retraction phase on the treadmill, whil
e no differences were found in the protraction angle. The vertical mov
ement of the hooves as well as the withers was smaller on the treadmil
l than on rubber ground. Treadmill belt velocity decreased by 9 % duri
ng the stance phase. This is supposed to be an important reason for th
e differences in biomechanics and kinematics between treadmill and ove
rground locomotion, which must be kept in mind when data obtained duri
ng treadmill locomotion have to be extrapolated to overground conditio
ns.