EXPRESSION OF PRELAMIN-A BUT NOT MATURE LAMIN-A CONFERS SENSITIVITY OF DNA BIOSYNTHESIS TO LOVASTATIN ON F9 TERATOCARCINOMA CELLS

The role of inhibition of prelamin A processing in the inhibition of D NA synthesis by lovastatin was examined by expressing prelamin A in F9 teratocarcinoma cells. These cells, normally lacking expression of th e A/C lamins, were transfected with constructs expressing either prela min A or mature lamin A and the effect of lovastatin on DNA biosynthes is was assessed. It was found that expression of prelamin A specifical ly conferred sensitivity to inhibition of DNA biosynthesis by lovastat in on F9 cells.