K. Wikiel et al., HYDROXYAPATITE MINERALIZATION AND DEMINERALIZATION IN THE PRESENCE OFSYNTHETIC PHOSPHORYLATED PENTAPEPTIDES, Archives of oral biology, 39(8), 1994, pp. 715-721
A constant composition (CC) method was used to compare the influence o
f statherin-like N-terminal 5-residue fragments having different amino
acids in the terminal position on hydroxyapatite (HAP) growth and dis
solution. The CC experiments were done in solutions containing 4.00 x
10(-4) mol/l calcium and 2.40 x 10(-4) mol/l phosphate. The solutions
used in the crystallization studies were supersaturated only with resp
ect to HAP (pH = 7.40, sigma(HAP) = 3.60). The CC dissolution studies
were done in solutions undersaturated with respect to HAP (pH = 6.00;
sigma(HAP) = -0.39). The HAP mineralization and demineralization proce
sses were markedly inhibited by the negatively charged pentapeptides.
Those containing a phosphorylated terminal residue inhibited dissoluti
on to a greater extent than the native statherin fragment having aspar
tate as the N-terminal residue. Strong dependencies of the degree of i
nhibition of growth and dissolution reaction rates on the extents of r
eaction were noted. As the reactions proceeded, the rate inhibition de
creased in the case of crystal growth and increased for dissolution.