CHARACTERIZATION AND CLONING OF A RECEPTOR FOR BMP-2 AND BMP-4 FROM NIH 3T3 CELLS

The bone morphogenetic proteins (BMPs) are a group of transforming gro wth factor beta (TGF-beta)-related factors whose only receptor identif ied to date is the product of the daf-4 gene from Caenorhabditis elega ns. Mouse embryonic NIH 3T3 fibroblasts display high-affinity (125I)-B MP-4 binding sites. Binding assays are not possible with the isoform I -125-BMP-2 unless the positively charged N-terminal sequence is remove d to create a modified BMP-2, I-125-DR-BMP-2. Cross-competition experi ments reveal that BMP-2 and BMP-4 interact with the same binding sites . Affinity cross-linking assays show that both BMPs interact with cell surface proteins corresponding in size to the type I (57- to 62-kDa) and type II (75- to 82-kDa) receptor components for TGF-P and activin. Using a PCR approach, we have cloned a cDNA from NIH 3T3 cells which encodes a novel member of the transmembrane serine/threonine kinase fa mily most closely resembling the cloned type I receptors for TGF-P and activin. Transient expression of this receptor in COS-7 cells leads t o an increase in specific I-125-BMP-4 binding and the appearance of a major affinity-labeled product of similar to 64 kDa that can be labele d by either tracer. This receptor has been named BRK-1 in recognition of its ability to bind BMP-2 and BMP-4 and its receptor kinase structu re. Although BRK-1 does not require cotransfection of a type II recept or in order to bind ligand in COS cells, complex: formation between BR K-1 and the BMP type II receptor DAF-4 can be demonstrated when the tw o receptors are eoexpressed, affinity labeled, and immunoprecipitated with antibodies to either receptor subunit. We conclude that BRK-1 is a putative BMP type I receptor capable of interacting with a known typ e II receptor for BMPs.