MOLECULAR KARYOTYPE ALTERATIONS INDUCED BY TRANSFORMATION IN ASPERGILLUS-NIDULANS ARE MITOTICALLY STABLE

Authors
XUEI XL SKATRUD PL
Citation
Xl. Xuei et Pl. Skatrud, MOLECULAR KARYOTYPE ALTERATIONS INDUCED BY TRANSFORMATION IN ASPERGILLUS-NIDULANS ARE MITOTICALLY STABLE, Current genetics, 26(3), 1994, pp. 225-227
Citations number
12
Categorie Soggetti
Genetics & Heredity
Journal title
Current genetics → ACNP
ISSN journal
01728083
Volume
26
Issue
3
Year of publication
1994
Pages
225 - 227
Database
ISI
SICI code
0172-8083(1994)26:3<225:MKAIBT>2.0.ZU;2-A
Abstract
Clamped homogeneous electric field (CHEF)-gel electrophoresis was used to define the electrophoretic molecular karyotype of Aspergillus nidu lans strain OC-1 before and after protoplast-based genetic transformat ion. The transforming DNA caused alterations in the molecular karyotyp es in all transformants examined. Rather dramatic changes were observe d in karyotypes, including apparent chromosome loss, massive size alte rations, and the appearance of large chromosomes. Changes in molecular karyotype were mitotically stable.