FLUORESCENCE REAGENTS FOR LABELING OF BIOMOLECULES .3. STUDY OF THE REACTIONS OF 2-SUBSTITUTED AND 4-SUBSTITUTED 9-ISOTHIOCYANATOACRIDINES WITH GLYCINE

Kinetics of nucleophilic addition reaction of 2- and 4-substituted 9-i sothiocyanatoacridines I - VII with glycine in buffered aqueous dimeth ylformamide has been studied. The addition products, N-(9-acridinylthi ocarbamoyl)glycines VIII - XIV, were characterized by IR, UV, H-1 NMR, mass and fluorescence spectra. Derivatives VIII, X and XII exhibited higher fluorescence intensity than the starting isothiocyanates; the h ighest fluorescence was found for the unsubstituted compound X. The re action mechanism is discussed on the basis of properties of the reacti on products and kinetic characteristics.