P. Koivisto et al., P-32 POSTLABELLING HPLC ASSAY REVEALS AN ENANTIOSELECTIVE ADDUCT FORMATION IN N7 GUANINE RESIDUES IN-VIVO AFTER 1,3-BUTADIENE INHALATION EXPOSURE/, Carcinogenesis, 18(2), 1997, pp. 439-443
We have established a protocol that allows qualitative and quantitativ
e determination of butadiene monoepoxide-DNA adducts formed as a resul
t of inhalation exposure to 1,3-butadiene. We observed that in this pa
rticular case irt vivo samples required extensive sample purification
to facilitate a low background, Sample preparation included a solid ph
ase extraction carried out with a strong anion exchange column and one
-dimensional ion exchange TLC, The ultimate analysis is based on rever
se phase HPLC with on-line radioactivity and UV detectors, The qualita
tive identification and quantitation is based on characterized markers
, which are used as external and internal standards, Modified 3'-dGMP
markers were used to control labelling efficiency, which varies, and m
odified 5'-dGMP markers were used as an optical standard to qualitativ
ely assign the products and to determine recovery of the sample prepar
ation, Using this method we were able to demonstrate, for the first ti
me, specific enantio- and regioisomeric adduct formation at the N7 pos
ition of guanine residues in liver DNA of rats inhalation-exposed to 1
,3-butadiene. The major adduct formed was the C-2 isomer derived from
the R enantiomer of butadiene monoepoxide, contributing 47% of all add
ucts formed at the N7 position of guanine, The relative proportions of
the remaining three other adducts detected were 22 (R C-l), 18 (S C-2
) and 14% (S C-l) respectively, Inhalation exposure to 200 p.p.m. for
5 days resulted in an alkylation level of 7.2 fmol/10 mu g DNA or 2.4
adducts/10(-7) normal nucleotides.