THE EFFECT OF SERUM AND COLOSTRUM IMMUNOGLOBULINS FROM BUFFALOS INFECTED WITH TOXOCARA-VITULORUM ON T-VITULORUM LARVAE IN-VITRO AND IN-VIVOIN MICE

Serum and colostrum were collected from adult buffalo cows naturally i nfected with Toxocara vitulorum. When injected into mice, the colostru m reduced the number of larvae of T. vitulorum that migrated in the mi ce. Injection of particularly the IgG-containing fraction but also the IEM-containing fraction of Sephadex G200-chromatographed colostrum al so passively protected mice. When incubated for 6 h in buffalo serum o r colostrum or fractions of these from Sephadex G200 and diethylaminoe thanol Sephadex, T. vitulorum larvae had their activity in vitro curta iled. When the larvae were then fed to mice, their ability to migrate was markedly inhibited as compared with that of larvae that had been i ncubated in fetal calf serum or in helminth-free sheep colostrum. Frac tions of serum and colostrum containing IgG(1) had greater inhibitory effects both on the larvae in vitro and on their subsequent migration in mice than did IgG(2)-containing fractions. IgM also inhibited the l arvae as 2-mercaptoethanol reduction of IgM in the IgM-containing peak eluted from Sephadex G200 reduced the inhibitory activity of this pea k, although the activity was not completely abrogated.