R. Kanda et al., EFFECTS OF COLCEMID CONCENTRATION ON CHROMOSOME ABERRATION ANALYSIS IN HUMAN-LYMPHOCYTES, Journal of radiation research, 35(1), 1994, pp. 41-47
As a part of technical improvements of chromosome aberration analysis
on human peripheral lymphocytes for biological radiation dosimetry, we
examined the optimal conditions for the use of colcemid in chromosome
preparation in order to obtain enough number of cells at metaphase in
the first cell division. When treated with colcemid at concentrations
below 0.01 mu g/ml from the beginning of culture, cultures harvested
at 48 hours had low mitotic indices. Colcemid treatment at 0.025 to 0.
05 mu g/ml during 48 hours resulted in high mitotic indices (8 to 15%)
and almost of the mitotic cells remaining in the 1 st cell division,
suggesting that this range of colcemid concentration was appropriate f
or continuous treatment with colcemid. We further examined the effect
of colcemid concentration on the quantitative consistency of the yield
s of radiation-induced chromosome aberration. Repeated experiments sho
wed that the yield of dicentrics and centric rings in the culture havi
ng colcemid at 0.025 mu g/ml concentration were larger than that at 0.
05 mu g/ml. These data indicate the importance of assuring the accurac
y of colcemid concentration in the lymphocyte culture for cytogenetic
radiation dosimetry.