ACTION OF DIETARY TRYPSIN, PRESSED COFFEE OIL, SILYMARIN AND IRON SALT ON 1,2-DIMETHYLHYDRAZINE TUMORIGENESIS BY GAVAGE

Sprague-Dawley male rats, 24 days of age, were placed on diets based o n a balanced ration as such and supplemented with Brazilian A rabica g reen coffee bean oil (0.10%), silymarin flavonolignans (0.10%), porcin e trypsin (2429 mu/g ration) and ferrous sulfate (0.24% Fe) for a peri od of 32 weeks. A portion of the controls was switched to the iron sal t diet at day 37 when 1,2-dimethylhydrazine was administered by gavage at a dosage of 20 mg/kg (base) and continued weekly for a total of W weeks. The colon and small intestinal adenocarcinoma numbers were dete rmined for each group of rats surviving the carcinogen treatment end c ompared with the respective controls by a statistical design based on Poisson distribution. The results indicate that the adenocarcinoma fre quencies of the colon, both total and occurrence in the proximal and d istal portions were significantly decreased in the groups fed coffee o il, silymarin group and trypsin. The colon tumor numbers for the iran salt-fed were in the control range except for a decrement in the dista l colon for rats on the diet from the start. Small intestinal adenocar cinoma scores with all supplemented diets did not differ significantly from the controls.