EVALUATION OF THE MERIFLUOR IMMUNOFLUORESCENT ASSAY FOR THE DETECTIONOF CRYPTOSPORIDIUM AND GIARDIA IN SODIUM-ACETATE FORMALIN-FIXED STOOLS

Authors
GRIGORIEW GA WALMSLEY S LAW L CHEE SL YANG J KEYSTONE J KRAJDEN M
Citation
Ga. Grigoriew et al., EVALUATION OF THE MERIFLUOR IMMUNOFLUORESCENT ASSAY FOR THE DETECTIONOF CRYPTOSPORIDIUM AND GIARDIA IN SODIUM-ACETATE FORMALIN-FIXED STOOLS, Diagnostic microbiology and infectious disease, 19(2), 1994, pp. 89-91
Citations number
9
Categorie Soggetti
Microbiology,"Infectious Diseases
Journal title
Diagnostic microbiology and infectious disease → ACNP
ISSN journal
07328893
Volume
19
Issue
2
Year of publication
1994
Pages
89 - 91
Database
ISI
SICI code
0732-8893(1994)19:2<89:EOTMIA>2.0.ZU;2-Q
Abstract
The Merifluor Cryptosporidium/Giardia direct immunofluorescent assay ( MFDFA) was used for the blinded detection of Cryptosporidium and Giard ia in 100 previously tested sodium acetate formalin (SAF)-preserved fe cal specimens. Examination for ova and parasites by formalin-ether con centration followed by iron-hematoxylin and Kinyon staining had demons trated Cryptosporidium(n = 32), Giardia (n = 20) and both Giardia and Cryptosporidium (n = 5). The MFDFA detected one additional specimen wi th Cryptosporidium and another with Giardia and was rapid, accurate, a nd simple to perform.