CAMP, ETHANOL, AND CO2 PRODUCTION WITH THE ADDITION OF D-GLUCOSE ANOMER TO STARVED YEAST CELLS

cAMP, ethanol, and CO2 production in starved yeast cells after the add ition of D-glucose anomer was measured and compared over a wide range of anomer concentrations. At 1.0 g/l or higher concentrations, the add ition of beta-D-glucose resulted in a higher cAMP peak, beta-D-glucose was more rapidly metabolized to ethanol and CO2 than alpha-D-glucose, although there was no notable difference in the uptake rates of the t wo anomers. At 0.4 g/l D-glucose anomer, the differences in cAMP and e thanol production rates for the two anomers were not significant. At 0 .2 g/l D-glucose anomer or lower concentrations, ethanol production wi th alpha-D-glucose was higher than that with beta-D-glucose. The uptak e rate of alpha-D-glucose was higher than that of beta-D-glucose at th is low concentration. (C) 1994 Academic Press, Inc.