As more sophisticated research is performed to refine fracture fixatio
n techniques for horses, it is important that normal values for the ge
ometric properties of the bones of the appendicular skeleton be determ
ined and that suitable controls be available. We evaluated the geometr
ic properties of total bone width, cortical bone width, and medullary
canal/trabecular bone width measured from 2 radiographic projections o
f equine long bones (humerus, radius, third metacarpal bone, femur, ti
bia, and third metatarsal bone) obtained from a general population of
horses. Measurements were performed on slices separated by intervals e
qual to 5% of the bone's length. Slices were then grouped into 5 regio
ns: proximal epiphysis, proximal part of the metaphysis, diaphysis, di
stal part of the metaphysis, and distal epiphysis. Results validated u
se of the contralateral bone as a control for assessing experimental m
odels or clinical cases. Of 858 homotypic slice comparisons between le
ft and right bones, significant (P less than or equal to 0.05) differe
nces were detected in 31 (3.6%) of the comparisons. Of 168 homotypic r
egion comparisons, significant differences were observed in 3 (1.8%) o
f the comparisons. The greatest variation between left and right bones
was observed in metaphyseal regions, areas with bony protuberances, a
nd regions with prominent bone superimposition. At a power of 0.8 for
the statistical tests performed in this study, the mean homotypic vari
ation of bones in each region is < 5.8% for the proximal epiphysis, 11
.3% for the proximal part of the metaphysis, 6.8% for the diaphysis, 1
2.2% for the distal part of the metaphysis, and 5.2% for the distal ep
iphysis.