DETECTION OF A CELL-LINE CONTAMINATED WITH HOG-CHOLERA VIRUS

Cell lines from the repository of the American Type Culture Collection were examined for possible contamination with bovine viral diarrhea v irus. During testing, hog cholera virus (HCV) was detected in the IB-R S-2 D10 porcine kidney cell line. This variant of HCV was avirulent fo r pigs and seldom induced delectable concentrations of antibody agains t reference viruses (HCV-Ames or bovine viral diarrhea virus-NY1) in s erum of inoculated pigs. Additionally, this variant of HCV did not con fer protection to pigs against virulent HCV. The contaminated cell lin e had been distributed to > 20 laboratories in the United States. The cell line was not used in field studies and has been destroyed.